2016/04/30

Trout lily survey

Why are the anther colours different in trout lilies?  This might be a question that enters your mind every spring if you enjoy exploring forests and watching ephemeral plants flower before the canopy closes over and the presence of biting insects dissuades you from entering the forest.

For those not sure what I am talking about, here is a photo I took this morning (in McCarthy Woods) of two trout lilies (Erythronium americanum) growing side-by-side that have different anther colours (red versus yellow).

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I dug the soil at the bottom to see if the plants are connected by the same root system or not.  I didn't find out but I think they may have developed from separate corms but could still be clones of each other.

Within-species variation in anther colour exists in several Erythronium and other plant species (due to genetic mutation), but the maintenance of this mutation in the wild and the ecological significance of this variation remains a mystery.  For instance, does anther colour vary geographically, and do pollinators and herbivores prefer to visit anther with certain colours?

Researcher in action
To address these fascinating research questions (and more), allow me to introduce my friend who is currently undertaking this project.  Here she is photographing a bee (I think).  She has created a website specifically for this project and welcomes people to help document anther colour variation in Eastern North America.  To participate in this citizen science project, please visit https://troutlilysurvey.wordpress.com/.

Yesterday we visited Gatineau Park to do a quick survey and found there were more trout lilies with red than yellow anther.

Trout Lily (Erythronium americanum)

Trout Lily (Erythronium americanum)

Trout Lily (Erythronium americanum)

Trout Lily (Erythronium americanum)

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A herbivore commonly found on trout lilies is the pollen-eating Red-necked False Blister Beetle (Asclera ruficollis), hence a good reason to see if the beetle prefers to visit flowers of a certain anther colour.  Here is a beetle on a flower with red anthers.

Trout Lily flower visited by false blister beetle and Helictidae bee
While the beetle was going for the nectar, an Halictidae bee also came for a visit.

We also spent a lot of time enjoying the nice weather and checking out various organisms.

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Syrphid fly

Mourning Cloak Butterfly (Nymphalis antiopa)
Mourning Cloak butterfly (Nymphalis antiopa)

Mourning Cloak Butterfly (Nymphalis antiopa)

Mourning Cloak Butterfly (Nymphalis antiopa)
This butterfly was more interesting in warming up than being bothered by me inching closer and closer.

Hepatica (Anemone sp.)
Gorgeous colours in these Hepatica flowers (Anemone sp.).

Hepatica (Anemone sp.)
I am getting more and more confused about the leaf differences between A. americana (round-lobed) and A acutiloba (sharp-lobed).  Now, I think most of the ones I saw were A. americana.

Dutchman's Breeches (Dicentra cucullaria)
Dutchman's Breeches (Dicentra cucullaria), which has a yellow "waistband" not present in the related squirrel-corn (D. canadensis).

Early Saxifrage (Micranthes virginiensis)
Early Saxifrage (Micranthes virginiensis)

Early Saxifrage (Micranthes virginiensis)

Bloodroot (Sanguinaria canadensis)
Bloodroot (Sanguinaria canadensis) - one of my favourite flowers.

Bloodroot (Sanguinaria canadensis)
The out of focus petals create a dream-like image.  My friend noticed shimmering on the inner part of the petals and wondered what is the significance of it.  That is what happens when you go outside and botanizing with fellow plant geeks.

Helictidae bee resting on Bloodroot (Sanguinaria canadensis)
An Halictidae resting on bloodroot petal.

Helictidae bee resting on Bloodroot (Sanguinaria canadensis)

My friend was hoping to establish a study site (for pollinator and herbivore observations) in Gatineau Park if the proportion of red to yellow anther is fairly similar.  However, she decided to do the observations at McCarthy Woods because I remembered seeing red and yellow anther colours there.  This morning, we met at McCarthy Woods and began setting up the pollinator observation arrays.  Here are some of my photos during the three hours where I recorded all the pollinator activities in the arrays.

Trout Lilies (Research array #2 - red pollen)
Trout Lilies (Research array #2 - red anther).

Andrena bee visiting trout lilies (Research array #3 - yellow pollen)
Andrena bee visiting trout lilies (Research array #1 - yellow anther).

Trout Lilies (Research array #3 - yellow pollen)
Trout Lilies (Research array #3 - yellow anther).

Trout Lilies (Research array #4 - red pollen)
Trout Lilies (Research array #4 - red anther).

Helictidae bee resting on Trout lily flower (Research array #4 - red pollen)
Halictidae bee resting on red anther (Research array #4 - red anther).

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Osmia bee (if I remembered correctly) collecting pollen (Research array #4 - red anther).

Zebra jumper (Salticus scenicus)
I noticed a couple Zebra jumpers (Salticus scenicus) in the array and wondered if they were there to catch pollinators.

Zebra jumper (Salticus scenicus)
Adorable!

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There were many moments when no pollinator was around, so my attention wondered off elsewhere.  For example, there was this Sharp-shinned Hawk (Accipiter striatus) in the distance looking at me (a cropped image photographed with my macro lens).

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There is a hawk-in-flight moment in this photo, do you see it?

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An 100% cropped image of the above photo.  This shows how sharp the macro lens is, especially because this crop is not in the center of the photo.

After the pollinator observation ended, I stayed behind to take more pictures.

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Bee (I forgot which one) on trout lily.

Trout Lily (Erythronium americanum)
I love my macro lens.

Trout Lily (Erythronium americanum)
Trout lily with yellow anther.

Trout Lily (Erythronium americanum)
Trout lily with red anther.

Carolina Spring Beauty (Claytonia caroliniana)
Carolina Spring Beauty (Claytonia caroliniana)

Carolina Spring Beauty (Claytonia caroliniana)

Red trillium (Trillium erectum)
Red trillium (Trillium erectum)

Mourning Cloak Butterfly (Nymphalis antiopa)
N. antiopa

Mourning Cloak Butterfly (Nymphalis antiopa)

Eastern Comma (Polygonia comma)
Eastern Comma (Polygonia comma) on ironwood (Ostrya virginiana).  I quite like the bokeh from this macro lens.

Eastern Comma (Polygonia comma)
Eastern Comma (Polygonia comma)

Eastern Comma (Polygonia comma)
Beautiful comma.

What a great way to finish the month of April.  Another month and I will be in Colorado working on my own research.  Yippee!

2 comments:

Tim said...

My initial thought on the colours of anthers was how old the flower was, but as I scrolled down, and saw your experiment setup, I think that would disprove my theory (you would've seen the colour change during the course of the experiment). My second guess is genetics...clearly, the soil properties are unlikely as one of your photos has two different colours growing from what is effectively the same soil.

PSYL said...

The timing of the anther dehiscing is only two days, so not enough time to change colour.

From one of the project's webpages (https://troutlilysurvey.wordpress.com/f-a-q-s/pollen-colour-v-anther-colour/): "Three of the anthers dehisce (reveal their pollen) on the first day of flower opening, while the other three wait until the second day. Presumably, this reduces the probability that a single pollinator will remove all of the flower’s pollen in a single go."

Questions remain about what effects this variation has on the interacting species (pollinators and herbivores) and on the plant itself (how the plants reproduce). All of which will be examined in this cool project.

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